Journal: Annals of Translational Medicine

Article Title: The immunoglobulin superfamily member 3 (IGSF3) promotes hepatocellular carcinoma progression through activation of the NF-κB pathway

doi: 10.21037/atm.2020.02.14

Figure Lengend Snippet: Primary antibodies with indicated concentration for WB, IHC, IF

Article Snippet: Primary antibodies with indicated concentration for WB, IHC, IF ( ). table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Dilution Company Cat No. WB IHC IF IGSF3 1:400 1:400 1:50 Bioss Bs-9196R PCNA 1:250 BOSTR BM0104 cyclin A 1:250 BOSTER A00700 cyclin E 1:400 BOSTER A00543-1 CDK1 1:400 BOSTER BM0027 CDK2 1:400 BOSTER PB0562 p-IKBα 1:400 Wanleibio WL03120 IKBα 1:400 Wanleibio WL02495 IKBα 1:200 Beyotime AF1282 p-NF-κB 1:400 Wanleibio WL02169 NF-κB 1:400 Wanleibio WL01273b NF-κB 1:200 Beyotime AF1234 GAPDH 1:800 BOSTER BM3876 Ki-67 1:200 BOSTER PB0065 Lamin B 1:1,000 Bioss bs-1840R β-action 1:800 Bioss bsm-33036M Open in a separate window caption a8 Primary antibodies with indicated concentration for WB, IHC, IF

Techniques: Concentration Assay

Journal: Frontiers in Immunology

Article Title: Multi-omics analysis and experimental validation of the value of monocyte-associated features in prostate cancer prognosis and immunotherapy

doi: 10.3389/fimmu.2024.1426474

Figure Lengend Snippet: CCNA2 identified as the best prognostic gene among monocyte-associated genes. (A, B) Functional analysis of monocyte-related prognostic genes. (C, D) GBM and RandomForest algorithms to screen key prognostic genes in the TCGA-PRAD dataset. (E, F) GBM and RandomForest algorithms to screen key prognostic genes in the GSE16560 dataset. (G, H) KM curves of CCNA2 and ACSM3 in the TCGA-PRAD dataset. (I, J) KM curves of CCNA2 and ACSM3 in the GSE16560 dataset.

Article Snippet: Next, CCNA2 antibody (BOSTER, PB9424) was applied dropwise to cover the tissue chip, which was left at room temperature for 2 hours.

Techniques: Functional Assay

Journal: Frontiers in Immunology

Article Title: Multi-omics analysis and experimental validation of the value of monocyte-associated features in prostate cancer prognosis and immunotherapy

doi: 10.3389/fimmu.2024.1426474

Figure Lengend Snippet: CCNA2 positively correlates with monocyte infiltration levels. (A) Correlation analysis of CCNA2 and monocyte infiltration levels. (B, C) Analysis of CCNA2 correlation with monocyte markers. (D) Mendelian randomization analysis of high HLA-DR expressing monocytes in relation to prostate cancer. (E–I) Single-cell analysis of the correlation between CCNA2 and immune cell infiltration.

Article Snippet: Next, CCNA2 antibody (BOSTER, PB9424) was applied dropwise to cover the tissue chip, which was left at room temperature for 2 hours.

Techniques: Expressing, Single-cell Analysis

Journal: Frontiers in Immunology

Article Title: Multi-omics analysis and experimental validation of the value of monocyte-associated features in prostate cancer prognosis and immunotherapy

doi: 10.3389/fimmu.2024.1426474

Figure Lengend Snippet: Functional analysis of CCNA2 in PRAD. (A) KEGG analysis of CCNA2 in PRAD. (B–J) GSEA analysis of CCNA2 in PRAD.

Article Snippet: Next, CCNA2 antibody (BOSTER, PB9424) was applied dropwise to cover the tissue chip, which was left at room temperature for 2 hours.

Techniques: Functional Assay

Journal: Frontiers in Immunology

Article Title: Multi-omics analysis and experimental validation of the value of monocyte-associated features in prostate cancer prognosis and immunotherapy

doi: 10.3389/fimmu.2024.1426474

Figure Lengend Snippet: CCNA2 has a high binding capacity to PRAD-targeted drugs. (A) Analysis of the binding capacity of CCNA2 to PD1 inhibitors. (B) Analysis of the binding capacity of CCNA2 to bicalutamide. (C) Analysis of the binding capacity of CCNA2 to enzalutamide. (D) Analysis of the binding capacity of CCNA2 to abiraterone.

Article Snippet: Next, CCNA2 antibody (BOSTER, PB9424) was applied dropwise to cover the tissue chip, which was left at room temperature for 2 hours.

Techniques: Binding Assay

Journal: Frontiers in Immunology

Article Title: Multi-omics analysis and experimental validation of the value of monocyte-associated features in prostate cancer prognosis and immunotherapy

doi: 10.3389/fimmu.2024.1426474

Figure Lengend Snippet: CCNA2 is highly expressed in PRAD and is associated with poor patient prognosis. (A, B) Differential expression of CCNA2 in PRAD. (C) Diagnostic predictive value of CCNA2 in PRAD. (D) KM curve of overall survival of CCNA2 in PRAD. (E) Prognostic predictive value of CCNA2 in PRAD.

Article Snippet: Next, CCNA2 antibody (BOSTER, PB9424) was applied dropwise to cover the tissue chip, which was left at room temperature for 2 hours.

Techniques: Quantitative Proteomics, Diagnostic Assay

Journal: iScience

Article Title: Preparation for mitosis requires gradual CDK1 activation

doi: 10.1016/j.isci.2025.112292

Figure Lengend Snippet: Cdk1 regulates Cyclin B accumulation in G2 phase (A) Schematic. Cdk1 and Plk1 activities that induce mitosis are detected at the S/G2 border and increase gradually throughout G2 phase. (B) Example of unsynchronized U2OS Cyclin B1-YFP cells growing on micropatterns. Lighter colors indicate higher Cyclin B1-YFP fluorescence. Arrows indicate addition of DMSO or inhibitors. Time lapse 30 min. Scale bar 20 μm. (C) Schematic of the in silico synchronization setup. DMSO-treated cells are synchronized in mitosis (top). The resulting Cyclin B1-YFP fluorescence curve (top right) is used to fit Cyclin B1-YFP fluorescence of individual cells before kinase inhibitor treatment (bottom). (D) Quantification of Cyclin B1-YFP fluorescence of cells growing on micropatterns, treated with DMSO or indicated kinase inhibitors after in silico synchronization as in (B) and (C). Graph shows average and standard error of Cyclin B1-YFP fluorescence (At least 15 cells per condition are showed. Data are representative of 4 additional independent experiments, except for Plk1 inhibitor that is present in 2 additional independent experiments). Please note that only Cyclin B1-YFP fluorescence after kinase inhibitor addition is plotted. (E) Quantification of Cyclin B1, Aurora A, and Aurora B immunofluorescence in 4N U2OS Cdk1as cells after 2 h treatment with 1NMPP1. At least 420 cells per condition are showed. Data are representative of 3 independent experiments. ∗∗∗ p < 0.001, using Student’s t test. Interquartile range and median values are indicated within violin plots.

Article Snippet: Rabbit polyclonal anti- Cyclin A2 , Atlas antibodies , Cat# HPA020626, RRID: AB_1847376.

Techniques: Fluorescence, In Silico, Immunofluorescence

Journal: iScience

Article Title: Preparation for mitosis requires gradual CDK1 activation

doi: 10.1016/j.isci.2025.112292

Figure Lengend Snippet: Cdk1 regulates transcription of mitotic factors (A) Quantification of Cyclin B1 immunofluorescence in 4N U2OS cells treated with Cdk1 inhibitor (RO3306), cycloheximide, or both for 2 h. The G2 population was separated in silico based on DAPI. At least 520 cells per condition are showed. Data are representative of 2 independent experiments; ∗∗∗ p < 0.001, using ANOVA. Interquartile range and median values are indicated within violin plots. (B) Schematic of the setup for RNA sequencing. HeLa cells were released after double thymidine synchronization. After 4.5 h Cdk1 inhibitor (RO3306) or DMSO was added. After 2 h cells were harvested for RNA sequencing analysis. (C and D) Volcano plots show log2 fold change between treated (RO3306) and non-treated (DMSO) normalized gene expressions (x axis), plotted versus the p value (y axis). Orange circles represent differentially expressed genes, and blue circles represent genes with similar expression (data from 3 independent experiments). (E) Schematic containing a selection of key components involved in direct, inner, and outer feedback regulating Cdk activity. (F) Gene Ontology and p values based on (C).

Article Snippet: Rabbit polyclonal anti- Cyclin A2 , Atlas antibodies , Cat# HPA020626, RRID: AB_1847376.

Techniques: Immunofluorescence, In Silico, RNA Sequencing, Expressing, Selection, Activity Assay

Journal: iScience

Article Title: Preparation for mitosis requires gradual CDK1 activation

doi: 10.1016/j.isci.2025.112292

Figure Lengend Snippet: Mathematical model of the cell cycle (A) Schematic representation of the mathematical model. (B) Model prediction (red line) of protein level dynamics after parameter estimation based on quantitative immunofluorescence of indicated proteins in U2OS cells from Akopyan et al. (blue dots). Please note that experimental data are only used until mitotic entry. The decrease of protein levels in mitosis is added to the model to mark mitosis upon full activation of Cdk1. (C) Model estimation of selected cell-cycle activities. Model time refers to number of calculation steps with a fixed duration. (D) Model prediction of Cyclin B level dynamics after inhibition of Cdk1, Cdk2, or Plk1.

Article Snippet: Rabbit polyclonal anti- Cyclin A2 , Atlas antibodies , Cat# HPA020626, RRID: AB_1847376.

Techniques: Immunofluorescence, Activation Assay, Inhibition

Journal: iScience

Article Title: Preparation for mitosis requires gradual CDK1 activation

doi: 10.1016/j.isci.2025.112292

Figure Lengend Snippet: Mitotic duration after Wee1 inhibition depends on when in G2 phase Wee1 inhibitors are added (A) Model prediction of G2 duration after Wee1 inhibition at different time points in G2 phase. Time when Wee1i added same as in (B) and (C). G2 phase starts approximately at model time 740, and 20 model time corresponds approximately to 30 min. How activities change relative to model time is visible in Figure 3 . (B) Model prediction of accumulated FoxM activity at mitotic entry after Wee1 inhibition at different time points in G2 phase. 100% denotes mitotic levels in absence of Wee1 inhibition. (C) Model prediction of Cyclin B level at mitotic entry after Wee1 inhibition at different time points in G2 phase. 100% denotes mitotic levels in absence of Wee1 inhibition. Striped line indicates apparent minimum Cyclin B levels at mitotic entry. (D and E) U2OS Cyclin B1-YFP cells were monitored by time-lapse microscopy upon addition of Wee1 inhibitors. Three different inhibitors were used: MK1775 (1 μM), PD0166285 (1 μM), and PD407824 (5 μM). (D) Duration of mitosis (x axis) is plotted versus Cyclin B1-YFP level at mitotic entry (y axis). 100% denotes median mitotic levels in absence of Wee1 inhibition. (E) Duration of mitosis (y axis) is plotted versus estimated time before mitosis should Wee1 inhibitors have not been added (x axis). The estimate is based on Cyclin B1-YFP accumulation of control cells in the same experiment ( Figures S3 E and S3F). 31–70 cells per condition are showed. The data are representative of 3 independent experiments.

Article Snippet: Rabbit polyclonal anti- Cyclin A2 , Atlas antibodies , Cat# HPA020626, RRID: AB_1847376.

Techniques: Inhibition, Activity Assay, Time-lapse Microscopy, Control

Journal: iScience

Article Title: Preparation for mitosis requires gradual CDK1 activation

doi: 10.1016/j.isci.2025.112292

Figure Lengend Snippet: Wee1 inhibition in G2 phase leads to a de-coupling of Cdk1 and Plk1 activities (A) Model prediction of Plk1 activity at mitotic entry after Wee1 inhibition at different time points in G2 phase. 100% denotes mitotic levels in absence of Wee1 inhibition. G2 phase starts approximately at model time 740, and 20 model time corresponds approximately to 30 min. How activities change relative to model time is visible in Figure 3 . (B) Model prediction of Cyclin B level, Plk1 level, Plk1 activity, and Cdk1 activity after inhibition of Wee1 at different time points in G2 phase. The dotted line in each graph represents the levels at mitotic entry when Wee1 is not artificially inhibited. Mit indicates mitosis. (C) Flow cytometry analysis of Plk1 levels and Plk1-mediated phosphorylation of TCTP (pTCTP) in mitotic U2OS cells. STLC (10 μM), to block cells in mitosis, was added with or without Wee1i for 2 h before harvest. Graph shows mitotic cells, gated as in Figure S5 A. Wee1i, cells treated with the Wee1 inhibitor MK1775 (1 μM) together with STLC; DMSO, cells treated with DMSO together with STLC. At least 2,000 mitotic cells were quantified per condition in two independent experiments.

Article Snippet: Rabbit polyclonal anti- Cyclin A2 , Atlas antibodies , Cat# HPA020626, RRID: AB_1847376.

Techniques: Inhibition, Activity Assay, Flow Cytometry, Phospho-proteomics, Blocking Assay

Journal: iScience

Article Title: Preparation for mitosis requires gradual CDK1 activation

doi: 10.1016/j.isci.2025.112292

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti- Cyclin A2 , Atlas antibodies , Cat# HPA020626, RRID: AB_1847376.

Techniques: Recombinant, Gene Expression, RNA Sequencing, Software

Journal: International Journal of Clinical and Experimental Pathology

Article Title: CCNA2 facilitates epithelial-to-mesenchymal transition via the integrin αvβ3 signaling in NSCLC

doi:

Figure Lengend Snippet: CCNA2 is upregulated in human NSCLC specimens. A. NSCLC expression in normal lung tissue and NSCLC specimens. Images were taken from the Human Protein Atlas (http://www.proteinatlas.org) online database. B. Oncomine data showing CCNA2 expression in normal vs. tumor of NSCLC. CCNA2 mRNA expression in the Hou lung and Su lung data set. C. Kaplan-Meier survival curves for the NSCLC patients. The overall survival times in the low (green, n=88) and high CCNA2 (red, n=87) groups, with a hazard ratio of 1.88 (95% confidence interval (CI) 1.2-2.94) and P=0.005. D. qPCR analysis of CCNA2 mRNA levels in various NSCLC cell lines relative to the normal bronchial epithelial cells BEAS-2B. PCR values were normalized to the levels of β-actin. Data were presented as the mean ± SD from three independent measurements. E. Western blot analysis of CCNA2 expression in different NSCLC cell lines. β-actin was used as loading controls.

Article Snippet: After membranes were blocked, they were incubated with monoclonal antibody CCNA2 (OriGene Technologies, USA), E-cadherin, N-cadherin, MMP-2 and MMP-9 (Abcam, UK), GAPDH and β-actin (Immunology Consultants Laboratory, USA) followed by incubation with horseradish peroxidase-conjugated IgGs (1:10000, Bioworld Biotechnology).

Techniques: Expressing, Western Blot

Journal: International Journal of Clinical and Experimental Pathology

Article Title: CCNA2 facilitates epithelial-to-mesenchymal transition via the integrin αvβ3 signaling in NSCLC

doi:

Figure Lengend Snippet: CCNA2 promotes H1975 cells migration and invasion in vitro. A. Wound healing assay. Confluent cell monolayers were wounded, and wound closure was monitored at 0 hour and 24 hour. Quantification of wound closure was calculated. B. Invasion assay. H1975 control or cells transfected with CCNA2 plasmid were subjected to a Transwell invasion assay. The invasive cells were stained with 1% crystal violet and counted. Data were collected from five fields in three independent experiments. Quantification of invasive cells per field was analyzed. For indicated comparisons, **P<0.01. C. In vitro wound healing assay with human H1975 cells after knock out with CCNA2 expression. Image was acquired at 0, 24 h time points after scratching (left panel). Quantification of wound closure was calculated (right panel). D. Representative staining of invasive potentials of human H1975 cells from in vitro Transwell assay (left panel). Quantification of invasive cells per field was analyzed (right panel). Statistical analyses were performed by the Student’s t test. The following symbols were used to indicate significant differences: **P<0.01.

Article Snippet: After membranes were blocked, they were incubated with monoclonal antibody CCNA2 (OriGene Technologies, USA), E-cadherin, N-cadherin, MMP-2 and MMP-9 (Abcam, UK), GAPDH and β-actin (Immunology Consultants Laboratory, USA) followed by incubation with horseradish peroxidase-conjugated IgGs (1:10000, Bioworld Biotechnology).

Techniques: Migration, In Vitro, Wound Healing Assay, Invasion Assay, Transfection, Plasmid Preparation, Transwell Invasion Assay, Staining, Knock-Out, Expressing, Transwell Assay

Journal: International Journal of Clinical and Experimental Pathology

Article Title: CCNA2 facilitates epithelial-to-mesenchymal transition via the integrin αvβ3 signaling in NSCLC

doi:

Figure Lengend Snippet: CCNA2 regulates EMT in NSCLC cells. A. Morphological changes by CCNA2 in H1975 and H1975 cells. B. Western blot (left panel) and qRT-PCR (right panel) shown down-regulated expression of E-cadherin and upregulated expression of N-cadherin in H1975-CCNA2 cells. C. In contrast, silencing of CCNA2 resulted in increased expression of E-cadherin and decreased expression of N-cadherin in H1975-CCNA2si cells. For indicated comparisons, **P<0.01.

Article Snippet: After membranes were blocked, they were incubated with monoclonal antibody CCNA2 (OriGene Technologies, USA), E-cadherin, N-cadherin, MMP-2 and MMP-9 (Abcam, UK), GAPDH and β-actin (Immunology Consultants Laboratory, USA) followed by incubation with horseradish peroxidase-conjugated IgGs (1:10000, Bioworld Biotechnology).

Techniques: Western Blot, Quantitative RT-PCR, Expressing

Journal: International Journal of Clinical and Experimental Pathology

Article Title: CCNA2 facilitates epithelial-to-mesenchymal transition via the integrin αvβ3 signaling in NSCLC

doi:

Figure Lengend Snippet: CCNA2 expression regulates integrin αvβ3/MMPs signaling in NSCLC cells. A. CCNA2 over-expression enhances integrin αvβ3 expression, whereas knockdown of CCNA2 inhibits integrin αvβ3 expression. B. Indicated H1975 cells were immunostained with rabbit anti-CCNA2 antibody (green) and DAPI (blue) for observation by laser confocal microscopy. C. The expression of MMP-2 and MMP-9 in H1975 NSCLC cells transfected with the vector expressing CCNA2 plasmid or CCNA2-siRNA was evaluated by immunoblotting.

Article Snippet: After membranes were blocked, they were incubated with monoclonal antibody CCNA2 (OriGene Technologies, USA), E-cadherin, N-cadherin, MMP-2 and MMP-9 (Abcam, UK), GAPDH and β-actin (Immunology Consultants Laboratory, USA) followed by incubation with horseradish peroxidase-conjugated IgGs (1:10000, Bioworld Biotechnology).

Techniques: Expressing, Over Expression, Confocal Microscopy, Transfection, Plasmid Preparation, Western Blot

Journal: International Journal of Clinical and Experimental Pathology

Article Title: CCNA2 facilitates epithelial-to-mesenchymal transition via the integrin αvβ3 signaling in NSCLC

doi:

Figure Lengend Snippet: Inhibitor of integrin αvβ3 reduces CCNA2-induced EMT, cell migration and invasion. A. H1975-CCNA2 cells were treated or not with 20 μM cilengitide during 24 h after which proteins were analyzed by western blot with specific antibodies against E-cadherin and N-cadherin. B. Analysis of migration potential from cilengitide and vehicle treated H1975-CCNA2 cells by a wound healing assay (left) and the quantification of wound closure (right). Bars show means ± SD of three independent experiments. Statistical analyses were performed by using the Student’s t test. C. Analysis of invasion potential from cilengitide and vehicle treated H1975-CCNA2 cells by a wound healing assay (left) and the quantification of wound closure (right). Bars show means ± SD of three independent experiments. Statistical analyses were performed by using the Student’s t test. For indicated comparisons, **P<0.01.

Article Snippet: After membranes were blocked, they were incubated with monoclonal antibody CCNA2 (OriGene Technologies, USA), E-cadherin, N-cadherin, MMP-2 and MMP-9 (Abcam, UK), GAPDH and β-actin (Immunology Consultants Laboratory, USA) followed by incubation with horseradish peroxidase-conjugated IgGs (1:10000, Bioworld Biotechnology).

Techniques: Migration, Western Blot, Wound Healing Assay

Journal: iScience

Article Title: Identification of two miRNAs regulating cardiomyocyte proliferation in an Antarctic icefish

doi: 10.1016/j.isci.2024.110128

Figure Lengend Snippet: Western blot analyses of some cardiac developmental regulators in C. hamatus and T. bernacchii hearts (A and B) Results of western blot analyses of 10 factors involved in cardiac development in the hearts of C. hamatus and T. bernacchii . The range of the used protein molecular weight markers is 10–180 kD. The corresponding molecular marker sizes besides each detected protein are as follows: 75KD for Bmp2, 35KD for Bmp4, 60KD for Bmp7, 60KD for Smad1, 45KD for Ccna2, 45KD for Nkx2.5, 60KD for Mef2a, 75KD for Tbx2, 45KD for Gata4 and 45KD for β-actin. (C) The relative abundance of the examined proteins derived from three biological replicates (Error bars indicate ±1 SEM). Levels with significant difference between C. hamatus and T. bernacchii are denoted with ‘∗’ ( p < 0.05, Student’s t test) and ‘∗∗’ ( p < 0.01).

Article Snippet: The primary antibodies (Nkx2.5 (Santa Cruz Bio, sc-376565); Tbx 2 (OriGene, TA344550); Gata4 (GeneTex, GTX113194); Bmp2 (HuaAn Bio, ER80602); Bmp4 (GeneTex, GTX128348); Bmp7 (Sigma, QC49491); Smad1/5/9 (Abcam, ab66737); Mef2A (GeneTex, GTX50398), Ccna2 (LSBio, LS-C31034), and β-actin antibody (HuaAn Bio, M1210-2) were diluted to appropriate concentrations with 1X PBST.

Techniques: Western Blot, Molecular Weight, Marker, Derivative Assay

Journal: iScience

Article Title: Identification of two miRNAs regulating cardiomyocyte proliferation in an Antarctic icefish

doi: 10.1016/j.isci.2024.110128

Figure Lengend Snippet: Hypoxia induced miR-458-3p/miR-144-5p downregulation and bmp2 upregulation in zebrafish heart and Antarctic fish heart (A) Quantitative RT-PCR showing up-regulated expression of bmp2 , reduced miR-458-3p and miR-144-5p in the hypoxia (DO = 1.0 ± 0.2 mg/L) acclimated zebrafishes’ heart (see Table S14 for details). Western blot analysis of Bmp2 in the DO = 1.0 ± 0.2 mg/L acclimated zebrafishes’ heart compared with that of the normoxia zebrafishes. (B) Quantitative RT-PCR showing upregulated expressions for the cell cycle-related genes cdc2 , cdc20 , cdc27 , ccnd1 , ccnd2 , ccna2 and ccnb1 in the heart of hypoxia acclimated zebrafish (see Table S15 for details). At least three biological replicates were conducted for each measurement. (C) Quantitative RT-PCR showing reduced expression of bmp2 and miR-458-3p in the conditioned red-blooded T. bernacchii s’ heart (see Table S16 for details). Western blot analysis of Bmp2 in the hypoxia conditioned red-blooded T. bernacchii s’ heart compared with that of the normoxia T. bernacchii . The relative abundance of the examined ( bmp2 ) and the gene of miR-458-3p derived from at least three biological replicates. Levels with significant difference between the hypoxia conditioned red-blooded T. bernacchii s’ heart and that of the normoxia T. bernacchii are denoted with ‘∗’ ( p < 0.05, Student’s t test). Error bars indicate ±1 SEM. The range of the used protein molecular weight markers is 10–180 kD. The corresponding molecular marker sizes besides each detected protein are as follows: 75KD for Bmp2 and 45KD for β-actin.

Article Snippet: The primary antibodies (Nkx2.5 (Santa Cruz Bio, sc-376565); Tbx 2 (OriGene, TA344550); Gata4 (GeneTex, GTX113194); Bmp2 (HuaAn Bio, ER80602); Bmp4 (GeneTex, GTX128348); Bmp7 (Sigma, QC49491); Smad1/5/9 (Abcam, ab66737); Mef2A (GeneTex, GTX50398), Ccna2 (LSBio, LS-C31034), and β-actin antibody (HuaAn Bio, M1210-2) were diluted to appropriate concentrations with 1X PBST.

Techniques: Quantitative RT-PCR, Expressing, Western Blot, Derivative Assay, Molecular Weight, Marker

Journal: iScience

Article Title: Identification of two miRNAs regulating cardiomyocyte proliferation in an Antarctic icefish

doi: 10.1016/j.isci.2024.110128

Figure Lengend Snippet:

Article Snippet: The primary antibodies (Nkx2.5 (Santa Cruz Bio, sc-376565); Tbx 2 (OriGene, TA344550); Gata4 (GeneTex, GTX113194); Bmp2 (HuaAn Bio, ER80602); Bmp4 (GeneTex, GTX128348); Bmp7 (Sigma, QC49491); Smad1/5/9 (Abcam, ab66737); Mef2A (GeneTex, GTX50398), Ccna2 (LSBio, LS-C31034), and β-actin antibody (HuaAn Bio, M1210-2) were diluted to appropriate concentrations with 1X PBST.

Techniques: Isolation, Transfection, In Vitro, Sequencing, Software